Facile Green Synthesis of Silver Nanoparticles Using Aqueous Leaf Extract of Origanum majorana with Potential Bioactivity against Multidrug Resistant Bacterial Strains

The high prevalence of nosocomial bacterial resistance contributes to significant mortality and morbidity around the world; thus, finding novel antibacterial agents is vital concern. Accordingly, present study attempted synthesize silver nanoparticles (AgNPs) using a green approach. Aqueous leaf extract Origanum majorana was used AgNPs efficiency against multidrug resistant strains detected. Characterization biogenic performed ultraviolet-visible spectrophotometry (UV-Vis), transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDX), Fourier transform infrared (FT-IR) analysis, diffraction analysis (XRD). disc diffusion method detect activity three multidrug-resistant strains. Preliminary UV-Vis revealed biosynthesis due peak formation at 374 nm, corresponding surface plasmon resonance (SPR) AgNPs. TEM micrographs detected synthesis small with an average particle size 26.63 nm. EDX presence following elements: oxygen (3.69%), carbon (2.93%), aluminum (1.29), silicon (2.83%), chloride (17.89%), (71.37%). Furthermore, XRD peaks 2 theta (?) degrees 38.18°, 44.36°, 64.35°, 77.54°, assigned planes crystals (111), (200), (220), (311), respectively. Collectively, these findings affirm potential physicochemical characteristics. antimicrobial indicated that Klebsiella pneumoniae strain most susceptible concentrations 50 100 µg/disk, inhibitory zones 21.57 24.56 mm, minimum concentration (MIC) found be 10 µg/mL, while bactericidal (MBC) 20 µg/mL. In conclusion, aqueous O. mediated sized AgNPs, effectiveness pathogens.